Enzymy i białka

Enzymy i białka

    Polimeraza TaqNova DNA - free RP1010

    Polimeraza TaqNova DNA-free (RP10)

    TaqNova DNA-free Polymerase is highly purified from DNA contaminants, enabling amplification of very conserved sequences (e.g. bacterial 16S rRNA region) without risk of false positive PCR results.
    RP10, RP1002, RP1010, RP1002-S

    DNaseMe dsDNAse EN33

    DNaseMe, dsDNase (EN33) – NEW

    DNaseMe is a 42.8 kDa recombinant endonuclease, derived from marine amphipods, expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. DNaseMe is highly active in a broad spectrum of temperatures, buffer conditions and pH. The specific activity is similar to bovine DNase I however, DNaseMe is characterized by higher stability in demanding reaction and storage conditions (e.g. high salt and detergent containing buffers, elevated temperature). These features make DNaseMe extremely useful for rapid and “RNA safe” degradation of genomic DNA, where absence of ribonucleases is critical to maintain the integrity of RNA.
    EN33, EN33-050, EN33-250, EN33-S

    Masterase, HL-dsDNase (EN31) – NEW

    Masterase is a 43.3 kDa heat-labile recombinant endonuclease, derived from a cold water eukaryotic organism, expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. Masterase can be easily inactivated by heat treatment in moderate temperatures. It is intended for applications where the presence of dsDNA influences experiments’ results in thermo-sensitive applications and it is extremely useful for rapid and safe purification of RNA or proteins samples from contaminating DNA.
    EN31, EN31-025, EN31-050, EN31-S, EN31-HC

    Saltonase, HL-Nuclease (EN32) – NEW

    Saltonase is a 28.4 kDa, cold-active, heat-labile recombinant endonuclease produced in E.coli. Saltonase originates from psychrophilic bacteria and effectively digests all types of DNA and RNA substrates in different buffer conditions and a broad range of temperatures. It is very active in demanding conditions, including low temperatures and environment with high salt content. These features make Saltonase extremely useful for removing undesired nucleic acids contamination during purification of proteins in laboratory and manufacturing workflows.
    EN32, EN32-050, EN32-250, EN32-S

    Polimeraza TaqNova Stoffel (RP810)

    TaqNova Stoffel DNA Polymerase is an universal and easy-to-use DNA polymerase, that works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’→3’ directions, it does not show a 3’→5’ and 5’→3’ exonuclease activity.
    RP810

    Lipaza L1 (EN54)

    Lipaza L1 to enzym, którego gen został wyizolowany z zimnolubnego szczepu Pseudomonas sp.

    Lipaza L1 jest enzymem o wysokiej aktywności lipolitycznej w zakresie temp. 20-50 °C, w zakresie pH=8,5-9,5 oraz wysokiej kompatybilności z wieloma rodzajami detergentów.

    EN55, EN55-005, EN55-050

    Lipaza L2 (EN55)

    Lipaza L2 to enzym, którego gen został wyizolowany z zimnolubnego szczepu Pseudomonas sp.

    Lipaza L2 jest enzymem o wysokiej aktywności lipolitycznej w zakresie temp. 20 – 50°C, w zakresie pH=8,5 – 9,5 oraz wysokiej kompatybilności z wieloma rodzajami detergentów.

    EN55, EN55-005, EN55-050

    Izomeraza L-arabinozowa (EN53)

    Izomeraza L-arabinozowa pochodzi z psychrotrofowej bakterii Arthrobacter sp. 22c wyizolowanej z próbek ziemi arktycznej.

    Rekombinantowy enzym katalizuje reakcje izomeryzacji L-arabinozy do L-rybulozy oraz D-galaktozy do D-tagatozy.

    EN53, EN53-001, EN53-005

    β-D-galaktozydaza S3 (EN51)

    β-D-galaktozydaza S3 to zaadaptowany do zimna enzym pochodzący z psychrotrofowej bakterii Arthrobacter sp. S3 wyizolowanej z próbek ziemi arktycznej. Katalizuje on reakcję hydrolizy laktozy do D-glukozy oraz D-galaktozy.

    Rekombinantowy enzym jest produkowany w prokariotycznym systemie ekspresyjnym Escherichia coli. β-D-galaktozydaza S3 charakteryzuję się wysoką aktywnością w niskich temperaturach w warunkach neutralnego pH.

    EN51, EN51-010, EN51-050

    RIBOPROTECT Hu-Mut Inhibitor RNaz (RT36)

    The RIBOPROTECT Hu-Mut is a genetically modified human placental RNase inhibitor expressed in Escherichia coli. The RIBOPROTECT Hu-Mut has significantly improved resistance to oxidation compared to the wild type human and porcine RNase inhibitors, and is stable at low DTT concentrations. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C by non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu-Mut is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
    RT36, RT36-020, RT36-100

    RIBOPROTECT Hu Inhibitor RNaz (RT35)

    RIBOPROTECT Hu jest rekombinowanym białkiem oczyszczonym ze szczepu E. coli niosącego plazmid z wklonownym genem kodującym ludzki inhibitor RNaz. Hamuje aktywność rybonukleaz takich jak RNaza A, RNaza B, RNaza C poprzez niekowalentne wiązanie w stosunku 1:1.
    RT35, RT35-020, RT35-100

    TRANSCRIPTME Reverse Transcriptase (RT32)

    TRANSCRIPTME jest rekombinowaną, termostabilną odwrotną transkryptazą M-MuLV (Moloney Murine Leukemia Virus) oczyszczaną z Escherichia coli. W obecności odpowiednich starterów enzym syntetyzuje komplementarną nić DNA wykorzystując jako matrycę RNA (synteza cDNA) lub jednoniciowe DNA (ssDNA).
    RT32, RT32-050, RT32-050