AMPLIFYME SG One-Step Universal RT-qPCR Mix (AM07) -produkt wycofany

Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on intercalating dsDNA binding dye chemistry: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
AM07, AM07-100, AM07-500

Dostępne opcje:
SKU Reakcje
AM07-500 500 reakcji Zapytaj o ofertę
AM07-100 100 reakcji Zapytaj o ofertę

The AMPLIFYME SG One-Step Universal RT-qPCR Mix is a convenient reaction mixture created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube.

The use of high-affinity antibody for hot-start polymerase ensures higher specificity, by reducing formation of primer-dimer structures. It allows to obtain wider dynamic range by removing competition for reaction reagents, it also leads to higher sensitivity and reproducibility.

Additionally, Mu-MLV Reverse Transcriptase and RNase Inhibitor and ROX solution are included in separate tubes.

Precisely optimized buffer components ensures optimal conditions for reverse transcriptase and hot-start polymerase activity. Additionally RNase Inhibitor protects RNA from unspecific RNases.

The AMPLIFYME SG One-Step Universal RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly-used Real-Time PCR platforms.

Features & advantages

  • Versatile – excellent for various PCR conditions using different Real-Time PCR instruments
  • Sensitive – reliable detection of low copies RNA targets
  • Reproducible – consistent amplification across a wide dynamic range
  • Specific – precisely selected anti-Taq antibody eliminates non-specific amplification
  • Fast – accurate detection of molecular targets in as fast as 40 minutes (with either two- or three-step cycling profiles)
  • Universal – reliable detection of RNA targets from broad range of samples
  • RT-qPCR
  • gene expression analysis
  • genetic profilling
  • miRNA profiling / quantification
  • mass screening
  • RNA viral pathogen detection
  • characterization of genetically modified organisms (GMO)

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