Polimeraza Phi29 to wysoce procesywna rekombinowana polimeraza o wyjątkowej aktywności przemieszczenia nici, która pozwala na wydajną izotermalną amplifikację DNA. Po przyłączeniu do matrycy enzym jest zdolny do insercji do 70 tysięcy par zasad. Polimeraza Phi29 posiada również korektorską aktywność egzonukleazy 3’→5’, działającą preferencyjnie na ssDNA lub RNA. Polimeraza Phi29 pozwala uzyskać niezwykle wysoką wydajność amplifikacji DNA nawet z niewielkich ilości matrycy.
EN20, EN20-10, EN20-010, EN20-50, EN20-050
Dostępne opcje:
| SKU | Units | ||
|---|---|---|---|
 | EN20-050 | 5000 U (10 U/µl) | Zapytaj o ofertę |
 | EN20-010 | 1000 U (10 U/µl) | Zapytaj o ofertę |
phi29 DNA Polymerase is a highly processive recombinant polymerase with exceptional strand displacement activity, which allows for highly efficient isothermal DNA amplification. The enzyme is capable of up to 70 thousands base insertions per binding event. The polymerase also possesses a 3’to 5’ proofreading exonuclease activity acting preferentially on ssDNA or RNA. Therefore 3′-modified primers are recommended.
Features
- Recombinant polymerase derived from the Bacillus subtilis phage phi29 and over-expressed in E. coli.
- Extremely processive polymerase, enabling amplification up to 70 kbp.
- Extremely high yields of amplified DNA can be obtained even from minute amounts of template.
- High-Fidelity polymerase – possesses a 3’-5’ exonuclease (proofreading) activity acting preferentially on ssDNA or RNA.
- Isothermal polymerase – no need for thermal cycling.
- Due to heat lability of the enzyme, it is possible to use the amplification products directly in the downstream applications
- Rolling Circle Amplification (RCA).
- In situ genotyping with padlock probes.
- Amplification of DNA for SNP and STR detection.
- Unbiased amplification of whole genome.
- DNA template preparation for sequencing.
- RNA-primed DNA amplification.
- Multiple displacement amplification (MDA).
- Cell-free amplification of DNA from single cells.
Polimeraza DNA phi29 (EN20) - produkt wycofany - Manual
Polimeraza DNA phi29 (EN20) - produkt wycofany - MSDS
Polimeraza DNA phi29 (EN20) - produkt wycofany - CoA L.846672
-
Circle-to-circle amplification coupled with microfluidic affinity chromatography enrichment for in vitro molecular diagnostics of Zika fever and analysis of anti-flaviviral drug efficacy
Autorzy Ruben R.G. Soares, Aleksandra Pettke, Agustín Robles-Remacho, Sahar Zeebaree, Sibel Ciftci, Marianna Tampere, Aman Russom, Marjo-Riitta Puumalainen, Mats Nilsson, Narayanan Madaboosi Produkty Ligaza DNA Tth (EN13), Polimeraza DNA phi29 (EN20) – produkt wycofany Rok 2021 Źródło Sensors and Actuators B: Chemical (336) -
Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue
Autorzy Daniel Gyllborg, Christoffer Mattsson Langseth, Xiaoyan Qian, Eunkyoung Choi, Sergio Marco Salas, Markus M Hilscher, Ed S Lein, Mats Nilsson Produkty Ligaza DNA Tth (EN13), Polimeraza DNA phi29 (EN20) – produkt wycofany, RIBOPROTECT Hu Inhibitor RNaz (RT35), TRANSCRIPTME Reverse Transcriptase (RT32) Rok 2020 Źródło Nucleic Acids Research
-
Circle-to-circle amplification coupled with microfluidic affinity chromatography enrichment for in vitro molecular diagnostics of Zika fever and analysis of anti-flaviviral drug efficacy
Autorzy Ruben R.G. Soares, Aleksandra Pettke, Agustín Robles-Remacho, Sahar Zeebaree, Sibel Ciftci, Marianna Tampere, Aman Russom, Marjo-Riitta Puumalainen, Mats Nilsson, Narayanan Madaboosi Produkty Ligaza DNA Tth (EN13), Polimeraza DNA phi29 (EN20) – produkt wycofany Rok 2021 Źródło Sensors and Actuators B: Chemical (336) -
Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue
Autorzy Daniel Gyllborg, Christoffer Mattsson Langseth, Xiaoyan Qian, Eunkyoung Choi, Sergio Marco Salas, Markus M Hilscher, Ed S Lein, Mats Nilsson Produkty Ligaza DNA Tth (EN13), Polimeraza DNA phi29 (EN20) – produkt wycofany, RIBOPROTECT Hu Inhibitor RNaz (RT35), TRANSCRIPTME Reverse Transcriptase (RT32) Rok 2020 Źródło Nucleic Acids Research